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Introduction to Human Geography
Title
Introduction to Human Geography
Personal Creator
R. Adam Dastrup
Description
This Pressbook, Introduction to Human Geography, covers the an introduction to geography as well as chapters on topics such as population and migration, cultural geography, political geography, sustainable development, food, water & agriculture, urban geography, environmental issues and living with disasters. You can access the OER here: https://humangeography.pressbooks.com/
Subjects
human geography, population geography, social and economic geography concepts, sustainable development, environmental impact, Disasters, Natural disasters, man-made disasters, Open Educational Resources (OER)
Local Identifiers
19-0079
SLCC Student Newspapers 2021-04-20
Title
SLCC Student Newspapers 2021-04-20
Description
The Globe, April 20, 2021, Online Edition
Subjects
boosters, Vaccinations, Virus, Presidents, immune system, blood, Department of Health, health, Health care, Salt Lake Community College, environmental impact, environmental protection, Environmental policy, Earth, Earth Day, public prosecutors, Lawyers, trials, Judicial proceedings, police, Homicides, murders, Public opinion, courthouses, courtrooms, courts, Judicial proceedings, verdict, Justice, Judges, Manslaughter
Local Identifiers
21-0648
2022 - Successful Transformation of pYES2 Plasmid into BL21 Leading to New Directions to Allow for Expression in Yeast - Poster Presentation
Title
2022 - Successful Transformation of pYES2 Plasmid into BL21 Leading to New Directions to Allow for Expression in Yeast - Poster Presentation
Description
For the PET Project at the InnovaBio lab, the goal was to grow a linked protein of PETase and MHETase in Brewer’s Yeast. This was because the result would be the breakdown of Polyethylene Terephthalate (or plastic) and have the waste product of ethanol that could be used in a variety of ways. However, when the yeast was grown, no protein bands of any kind appeared on an SDS-PAGE gel or Western Blot after the yeast were lysed and sonicated. With the expectation of seeing the protein expressed after being lysed and sonicated, the project made a transition from yeast to Escherichia coli with the same pYES2 plasmid as it was compatible and could also be transformed into E. coli. Here, I present the pYES2 plasmid in BL21 E. coli. SDS-PAGE gels of the induced E. coli show that we were successful at lysing and sonicating but lack the expression of the protein. Double digest and PCR of purified plasmid show that the protein is present in the E. coli. These findings show that the sequence was successfully inserted into the pYES2 plasmid. However, further testing is needed to verify sequencing of the protein as well as to determine why galactose is not causing induction.
Subjects
biotechnology, plastic, Plastics, bioengineering, biodeterioration, biodegradable, proteins, degradation, environmental control, environmental engineering, environmental impact, environmental protection, student projects
Local Identifiers
22-0153